waters 蛋(dan)白質(zhi)反相(xiang)色譜(pu)柱(zhu)
蛋(dan)白質(zhi)反相(xiang)色譜(pu)分(fen)析(xi)是(shi)壹(yi)種(zhong)基於蛋(dan)白質(zhi)在(zai)溶(rong)液中(zhong)的相(xiang)對疏水性(xing)的分(fen)離(li)技術(shu)，分(fen)析(xi)時使(shi)用(yong)裝填(tian)有(you)短鏈(lian)鍵合(he)相(xiang)的大(da)孔(kong)徑顆(ke)粒(li)（例(li)如(ru)，300Å）的色(se)譜(pu)柱。

waters 蛋(dan)白質(zhi)反相(xiang)色譜(pu)柱(zhu)

反(fan)相(xiang)色譜(pu)

waters 蛋(dan)白質(zhi)反相(xiang)色譜(pu)柱(zhu)是(shi)壹種基於蛋(dan)白質(zhi)在(zai)溶(rong)液中(zhong)的相(xiang)對疏水性(xing)的分(fen)離(li)技術(shu)，分(fen)析(xi)時使(shi)用(yong)裝填(tian)有(you)短鏈(lian)鍵合(he)相(xiang)的大(da)孔(kong)徑顆(ke)粒(li)（例(li)如(ru)，300Å）的色(se)譜(pu)柱。如(ru)果使(shi)用(yong)離子對(dui)試(shi)劑(ji)（例(li)如(ru)0.1% TFA）來zui大(da)限(xian)度(du)減少不良(liang)離子幹(gan)擾，通常利(li)用(yong)逐漸升(sheng)高(gao)的有(you)機(ji)溶劑(ji)濃(nong)度梯(ti)度來控(kong)制(zhi)分(fen)離(li)。壹(yi)般來說(shuo)，洗(xi)脫(tuo)順(shun)序取決(jue)於蛋(dan)白質(zhi)或蛋(dan)白質(zhi)亞基的疏水性(xing)，疏水性(xing)zui弱的蛋(dan)白質(zhi)將首(shou)先(xian)洗(xi)脫(tuo)出(chu)來。此(ci)外(wai)，顆(ke)粒(li)組(zu)成(cheng)（矽(gui)膠(jiao)vs雜化(hua)顆(ke)粒(li)）、孔(kong)徑、鍵合(he)相(xiang)類型(xing)和密(mi)度，以及分(fen)離(li)條(tiao)件(jian)（例(li)如(ru)梯(ti)度(du)持續時間、分(fen)離(li)溫(wen)度(du)、流速）等(deng)等(deng)對(dui)於實(shi)現(xian)滿(man)足(zu)應用(yong)要求(qiu)的分(fen)離(li)而(er)言(yan)也(ye)非常重(zhong)要。

BEH顆(ke)粒(li)技術(shu)

專為蛋(dan)白質(zhi)分(fen)離(li)設(she)計(ji)

分(fen)離(li)大(da)分(fen)子生(sheng)物化合物的難(nan)度(du)較(jiao)大。20多年來，沃(wo)特(te)世反(fan)相(xiang)色譜(pu)柱(zhu)在(zai)蛋(dan)白質(zhi)分(fen)離(li)方(fang)面的性(xing)能(neng)優勢(shi)為(wei)色譜工作者提供了(le)有(you)力(li)支持。面(mian)對(dui)棘手的蛋(dan)白質(zhi)分(fen)離(li)難(nan)題，沃特(te)世亞(ya)乙(yi)基橋雜化(hua)(BEH)顆(ke)粒(li)技術(shu)有助(zhu)於用(yong)戶(hu)突(tu)破(po)使(shi)用(yong)*矽(gui)膠(jiao)基質填(tian)料(liao)色譜(pu)柱(zhu)時的性(xing)能(neng)局(ju)限(xian)。

• 300 Å孔(kong)徑

• C4鍵合(he)相(xiang)

• zui大限(xian)度(du)減(jian)少不良(liang)次級(ji)相(xiang)互作(zuo)用(yong)

• zui大限(xian)度(du)減(jian)少高(gao)溫分(fen)離(li)時的可(ke)檢(jian)出(chu)殘留(liu)

• 可(ke)重(zhong)現(xian)的蛋(dan)白質(zhi)分(fen)離(li)

• 采用(yong)成熟(shu)的*鍵合(he)工藝

• 在(zai)低(di)pH和高(gao)溫條件(jian)下(xia)穩(wen)定(ding)

• 采用(yong)多種(zhong)蛋(dan)白質(zhi)混合(he)物(wu)進行(xing)質(zhi)控(kong)測(ce)試(shi)

• 出(chu)色的批次(ci)間(jian)重(zhong)現(xian)性(xing)

• 提供適(shi)用(yong)於UPLC和微升(sheng)級(ji)UPLC應用(yong)的1.7 μm顆(ke)粒(li)規格(ge)

• 使(shi)用(yong)納流(liu)和微流LC-MS進行(xing)蛋(dan)白質(zhi)分(fen)離(li)

  150 μm iKey分(fen)離(li)裝(zhuang)置的“即(ji)插(cha)即(ji)用(yong)"式(shi)設(she)計(ji)和無(wu)接頭(tou)流(liu)路(lu)連接大大簡化(hua)了(le)采用(yong)微流LC-MS的蛋(dan)白質(zhi)分(fen)析(xi)方(fang)法。與1 mm內徑的UPLC相(xiang)比(bi)，iKey將分(fen)析(xi)靈敏(min)度提高(gao)了(le)多達40倍。

• CQUITY UPLC M-Class色譜(pu)柱(zhu)專為低(di)擴(kuo)散(san)納(na)流(liu)LC而(er)設(she)計，適用(yong)於納流(liu)LC-MS蛋(dan)白質(zhi)分(fen)析(xi)。納(na)升(sheng)級(ji)至微升(sheng)級(ji)LC/MS ACQUITY UPLC M-Class色譜柱和捕(bu)集(ji)柱(zhu)可(ke)在(zai)15000 psi的UPLC條(tiao)件(jian)下(xia)進行(xing)納(na)升(sheng)級(ji)和微升(sheng)級(ji)分(fen)離(li)，充(chong)分(fen)利(li)用(yong)亞2 μm顆(ke)粒(li)技術(shu)的分(fen)離(li)能(neng)力(li)。

• BioSuite pPhenyl反相(xiang)色譜(pu)(RPC) HPLC色(se)譜(pu)柱

  BioSuite RPC色譜(pu)柱產(chan)品(pin)中裝填有與pH穩定的甲基丙烯酸酯(zhi)類(lei)聚合物(wu)樹(shu)脂(zhi)鍵合(he)的苯基(pPhenyl)填料(liao)。pPhenyl基質的孔(kong)徑為(wei)1000 Å，可(ke)分(fen)析(xi)分(fen)子量達5 000 000道爾頓的蛋(dan)白質(zhi)。

  pPhenyl RPC填料(liao)提供裝(zhuang)填於5 x 150 mm色譜(pu)柱(zhu)的規格(ge)，適用(yong)於“實(shi)驗室(shi)規模(mo)"分(fen)離(li)；還(hai)提供裝(zhuang)填於2.0 x 75 mm色譜(pu)柱(zhu)的規格(ge)，適用(yong)於窄(zhai)徑HPLC和LC-MS應用(yong)。

• Symmetry300 C4 HPLC和UHPLC色(se)譜柱(zhu)

  Symmetry300 C4是(shi)采用(yong)超純(chun)有機試(shi)劑(ji)合(he)成(cheng)的*矽(gui)膠(jiao)基質材料(liao)，純度(du)*且(qie)矽(gui)醇(chun)活性(xing)極(ji)低(di)，對(dui)肽(tai)和蛋(dan)白質(zhi)的分(fen)離(li)和回收效果(guo)較佳。

• 300 Å孔(kong)徑，適(shi)用(yong)於肽(tai)和蛋(dan)白質(zhi)應用(yong)

• *封(feng)端(duan)，zui大限(xian)度(du)減(jian)少(shao)不(bu)良次(ci)級相(xiang)互作(zuo)用(yong)

• 相(xiang)較於Waters BEH C4, 300 Å雜化(hua)填料(liao)，為分(fen)離(li)選(xuan)擇(ze)性帶(dai)來更(geng)多選(xuan)擇(ze)

• 采用(yong)肽(tai)標準品進行(xing)QC測(ce)試(shi)，可(ke)確(que)保出(chu)色的批次(ci)間(jian)*性(xing)

• Delta-Pak色譜柱(zhu)

• Delta-Pak HPLC色(se)譜(pu)柱(zhu)基於高(gao)度穩定(ding)、鍵合(he)、封(feng)端(duan)的球(qiu)形(xing)5 μm或15 μm 100％矽(gui)膠(jiao)基質顆(ke)粒(li)。該(gai)系列(lie)色(se)譜柱(zhu)有100 Å和300 Å兩種(zhong)孔(kong)徑 - 填(tian)充(chong)C4或C18鍵合(he)相(xiang)，可(ke)滿(man)足(zu)不同的選(xuan)擇(ze)性和保留性(xing)需(xu)求(qiu)。提供不(bu)同的15 μm小(xiao)柱(zhu)和色(se)譜柱(zhu)配(pei)置，能夠(gou)實(shi)現(xian)*且(qie)可(ke)預(yu)測的毫克(ke)級(ji)到克(ke)級(ji)純化放(fang)大(da)。

• 訂(ding)貨信(xin)息(xi)

  貨號(hao)	描(miao)述
  186007567	ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 50 mm, 1/pkg
  186007569	ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pkg
  WAT046885	DeltaPak C4 Guard Column, 300Å, 5 µm, 3.9 mm X 20 mm, 2/pkg
  186007495	ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pkg
  WAT011812	DeltaPak C4 Column, 300Å, 15 µm, 3.9 mm X 300 mm, 1/pkg
  WAT036865	DeltaPak C4 Guard-Pak Insert, 300Å, 5 µm, 10/pkg
  186007493	ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pkg
  186007494	ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pkg
  186005589	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 50 mm, 1/pkg
  186004899	ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg
  186006549	ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg
  186002156	BioSuite Phenyl Column, 1000Å, 10 µm, 2 mm X 75 mm, 1/pkg
  186005590	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 100 mm, 1/pkg
  186005591	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 150 mm, 1/pkg
  WAT011794	DeltaPak C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186004497	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg
  186004623	ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg
  186004496	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg
  WAT011796	DeltaPak C4 Column, 100Å, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186004495	ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg
  WAT011807	DeltaPak C4 Column, 100Å, 15 µm, 3.9 mm X 300 mm, 1/pkg
  186002157	BioSuite Phenyl Column, 1000Å, 10 µm, 4.6 mm X 75 mm, 1/pkg
  WAT046875	DeltaPak C4 Guard Column, 100Å, 5 µm, 3.9 mm X 20 mm, 2/pkg
  186002158	BioSuite Phenyl Column, 1000Å, 13 µm, 21.5 mm X 150 mm, 1/pkg
  WAT011813	DeltaPak C4 Prep Column, 300Å, 15 µm, 7.8 mm X 300 mm, 1/pkg
  WAT011814	DeltaPak C4 Prep Column, 300Å, 15 µm, 19 mm X 300 mm, 1/pkg
  186000277	Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg
  186000278	Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 100 mm, 1/pkg
  186000279	Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg
  WAT011810	DeltaPak C4 Prep Column, 100Å, 15 µm, 30 mm X 300 mm, 1/pkg
  WAT011669	DeltaPak C4 Radial-Pak Cartridge, 300Å, 15 µm, 47 mm X 300 mm, 1/pkg
  WAT037700	DeltaPak C4 Radial-Pak Cartridge, 300Å, 15 µm, 40 mm X 100 mm, 1/pkg
  WAT025848	DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 8.0 mm X 100 mm, 1/pkg
  186000286	Symmetry C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg
  186000285	Symmetry C4 Column, 300Å, 5 µm, 2.1 mm X 150 mm, 1/pkg
  WAT037851	DeltaPak C4 Radial-Pak Guard, 300Å, 15 µm, 40 mm X 10 mm, 2/pkg
  WAT038524	DeltaPak C4 Radial-Pak Guard, 100Å, 15 µm, 25 mm X 10 mm, 2/pkg
  186000283	Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg
  WAT038526	DeltaPak C4 Radial-Pak Guard, 300Å, 15 µm, 25 mm X 10 mm, 2/pkg
  186000282	Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg
  186000281	Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 75 mm, 1/pkg
  186000280	Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg
  WAT038508	DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 25 mm X 100 mm, 1/pkg
  WAT034225	Shodex DE-G Guard Column, 4.6 x 10 mm
  WAT011808	DeltaPak C4 Prep Column, 100Å, 15 µm, 7.8 mm X 300 mm, 1/pkg
  WAT011809	DeltaPak C4 Prep Column, 100Å, 15 µm, 19 mm X 300 mm, 1/pkg
  WAT011633	DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 47 mm X 300 mm, 1/pkg